YOU ARE HERE: Minipig » Sperm mediated gene transfer

Sperm mediated gene transfer

Pigs with expression of transgenes may be generated by sperm mediated gene transfer (SMGT).


The SMGT technique consists of the following steps (see schematic presentation here):

  • Collection of sperm cells from many different boars
  • Evaluation of sperm cell quality: motility, survival rate, morphology and ability to take up exogenous DNA
  • Preparation of transgene – linearized DNA is used for uptake in sperm cells
  • Removal of seminal fluid and mix with transgene – DNA uptake
  • Transfer of transgenic sperm cells to recipient sow by artificial insemination
  • Birth and molecular test of transgenic offspring

Contact

Questions and requests about service should be send to Knud Larsen

Email: knud.larsen@agrsci.dk, Phone:  +45 8999 1827          

Comments on content: 
Revised 2010.04.13