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Sperm mediated gene transfer

Pigs with expression of transgenes may be generated by sperm mediated gene transfer (SMGT).


The SMGT technique consists of the following steps (see schematic presentation here):

  • Collection of sperm cells from many different boars
  • Evaluation of sperm cell quality: motility, survival rate, morphology and ability to take up exogenous DNA
  • Preparation of transgene – linearized DNA is used for uptake in sperm cells
  • Removal of seminal fluid and mix with transgene – DNA uptake
  • Transfer of transgenic sperm cells to recipient sow by artificial insemination
  • Birth and molecular test of transgenic offspring

Contact

Questions and requests about service should be send to Knud Larsen

Email: knud.larsen@agrsci.dk, Phone:  +45 8999 1827          

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Revised 2011.11.03

Aarhus University
Nordre Ringgade 1
DK-8000 Aarhus C

Email: au@au.dk
Tel: +45 8715 0000
Fax: +45 8715 0201

CVR no: 31119103

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